bladder tumor cell line t24  (ATCC)

Journal: Oncology Letters

Article Title: LAPTM4B promotes the progression of bladder cancer by stimulating cell proliferation and invasion

doi: 10.3892/ol.2021.13026

Figure Lengend Snippet: LAPTM4B expression was effectively knocked down in both the T24 and 5637 human bladder cancer cell lines from the transfection of LAPTM4B shRNA. (A) Quantitative PCR and (B) western blot analysis revealed the significantly reduced mRNA and protein expression levels of LAPTM4B following transfection with target shRNA in the T24 and 5637 cell lines, respectively. The results are presented as the mean ± SD. *P<0.05. LAPTM4B, lysosome-associated transmembrane protein 4β; sh, short hairpin.

Article Snippet: The T24 and 5637 bladder tumor cell lines were purchased from American Type Culture Collection.

Techniques: Expressing, Transfection, shRNA, Real-time Polymerase Chain Reaction, Western Blot

Journal: Oncology Letters

Article Title: LAPTM4B promotes the progression of bladder cancer by stimulating cell proliferation and invasion

doi: 10.3892/ol.2021.13026

Figure Lengend Snippet: LAPTM4B promotes the proliferation, and migration and invasion of bladder cancer cells in vitro . (A) Representative images of colony formation assays in the T24 and 5637 cell lines transfected with control or LAPTM4B shRNA. (B) The results of MTT assays showed the inhibition of cell proliferation caused by knockdown of LAPTM4B. (C) Wound healing assays were performed in the T24 and 5637 cells transfected with control or LAPTM4B-shRNA and images were captured at 0 and 24 h time points. (D) Transwell invasion assays were performed in cells transfected with siRNA control or LAPTM4B in the T24 and 5637 cell lines and the number of invasive cells were quantified and analyzed statistically. The results are presented as the mean ± SD. *P<0.05. OD, optical density; sh, short hairpin.

Article Snippet: The T24 and 5637 bladder tumor cell lines were purchased from American Type Culture Collection.

Techniques: Migration, In Vitro, Transfection, Control, shRNA, Inhibition, Knockdown

Journal: Open Medicine

Article Title: Superenhancer–transcription factor regulatory network in malignant tumors

doi: 10.1515/med-2021-0326

Figure Lengend Snippet: The presence of SE–TF regulatory network consisting of SMAD3, ETS1, and HOXB2 in T24 cells. (a) H3K27ac intensity of SMAD3 in eight common malignant tumor cell lines analyzed by ChIP-PCR. (b) Expression of core TFs of bladder cancer (SMAD3, ETS1, and HOXB2) in T24 cells determined by RT-qPCR. (c) Silencing and overexpression efficiency of SMAD3, ETS1, and HOXB2 in T24 cells determined by western blot analysis. (d) Overexpression efficiency of SMAD3, ETS1, and HOXB2 in T24 cells determined by RT-qPCR. (e) Expression of other two TFs in T24 cells following silencing of any bladder cancer TFs determined by western blot analysis. (f) Expression of other two TFs in T24 cells following overexpression of any bladder cancer TFs determined by RT-qPCR. (g) Expression of other TFs in T24 cells following intervention with two types of bladder cancer TFs determined by western blot analysis. (h) Expression of other TFs in T24 cells following intervention with two types of bladder cancer TFs determined by RT-qPCR. (i) The binding of each TF to the promoter regions of the other two TFs analyzed by ChIP-PCR. * p < 0.05. The experiment was conducted three times independently.

Article Snippet: Human gastric malignant tumor cell line MKN45, human renal malignant tumor cell line 786-M1A, human esophageal squamous cell line KYSE140, human colorectal malignant tumor cell line HCT116, human bladder malignant tumor cell line T24, and human breast malignant tumor cell line 76NF2V, small cell lung malignant tumor cell line COR-L311, and human liver malignant tumor cell line HepG2 were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).

Techniques: Expressing, Quantitative RT-PCR, Over Expression, Western Blot, Binding Assay

Journal: Open Medicine

Article Title: Superenhancer–transcription factor regulatory network in malignant tumors

doi: 10.1515/med-2021-0326

Figure Lengend Snippet: The SE–TF regulatory network consisting of SMAD3, ETS1, and HOXB2 promotes the malignant phenotype of bladder cancer cells. (a) Migration, invasion, and proliferation of T24 cells following overexpression or silencing of SMAD3, ETS1, and HOXB2 measured by Transwell and CCK-8 assays. (b) Migration, invasion, and proliferation of T24 cells following transfection with sh-SMAD3, sh-SMAD3 + pcDNA3.1-ETS1, sh-SMAD3 + pcDNA3.1-HOXB2, or sh-SMAD3 + pcDNA3.1-ETS1 + pcDNA3.1-HOXB2 measured by Transwell and CCK-8 assays. * p < 0.05. The experiment was conducted three times independently.

Article Snippet: Human gastric malignant tumor cell line MKN45, human renal malignant tumor cell line 786-M1A, human esophageal squamous cell line KYSE140, human colorectal malignant tumor cell line HCT116, human bladder malignant tumor cell line T24, and human breast malignant tumor cell line 76NF2V, small cell lung malignant tumor cell line COR-L311, and human liver malignant tumor cell line HepG2 were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).

Techniques: Migration, Over Expression, CCK-8 Assay, Transfection

Journal: Open Medicine

Article Title: Superenhancer–transcription factor regulatory network in malignant tumors

doi: 10.1515/med-2021-0326

Figure Lengend Snippet: Core transcription factors in eight malignant tumor cell lines

Article Snippet: Human gastric malignant tumor cell line MKN45, human renal malignant tumor cell line 786-M1A, human esophageal squamous cell line KYSE140, human colorectal malignant tumor cell line HCT116, human bladder malignant tumor cell line T24, and human breast malignant tumor cell line 76NF2V, small cell lung malignant tumor cell line COR-L311, and human liver malignant tumor cell line HepG2 were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).

Techniques: